Technology

 1 ANIMAL MODELS (mouse and rat)

– Renal diseases

  • Acute kidney injury (toxic, ischemic, septic, pre-renal)
  • Chronic kidney disease
  • Fibrosis (renal mass reduction, ureteral obstruction)

– Hypertension

  • Genetic
  • Induced (DOCA-salt, L-NAME, aortic coarctation, AII pumps, renal artery clamping, Goldblatt)

– Diabetes

  • Genetic
  • Induced (streptozotocin)

– Other models

  • Hepatic diseases
  • Tumour models
  • Wound healing

2 ISOLATED ORGAN

– In situ & ex vivo perfused kidney

– Isolated artery contraction (wire myograph)

– Isolated heart and atria

3 RENAL FUNCTION

– Glomerular filtration rate

  • Inulin clearance
  • Creatinine clearance
  • Sinistrin clearance

– Renal plasma flow

  • Laser doppler
  • PAH clearance
  • Microspheres

– Electrolyte balance

– Urinary and tissue biomarkers of renal injury

– Furosemide test

4 CARDIOVASCULAR FUNCTION

– Arterial pressure and heart rate

  • Telemetry
  • Sphygmomanometry
  • Canulation

– Vascular function and stiffness

  • Retinography
  • ABI Index, PWV, IMT
  • pAIX, cAIX, AASSI

– Electrocardiography and echocardiography

– Angiogenesis

5 CELLULAR & MOLECULAR  BIOLOGY

– Cell culture

  • Primary cultures
  • Cell lines

– Flow cytometry

– Protein expression (Western blot, ELISA)

– mRNA analysis (qRT-PCR, digital RT-PCR)

– Live cell light and fluorescence microscopy

– Intracellular signalling

– Cell viability and cell cycle

 -Cell death (apoptosis, necrosis, necroptosis)

– Cell contraction, migration and invasion

– Oxidative stress and inflammation

6 HISTOPATHOLOGY

– Basic stains

– Immunohistochemistry

– Immunofluorescence

7 PROTEOMICS & METABOLOMICS

– 2D-DIGE

– MALDI-TOF

– TOF-TOF instruments

– MS/MS

– PMF

– MALDI biotypes

– MALDI imaging

– HPLC, UPLC, FPLC

8 TOXICOLOGY

– In silico

– Preclinical

– Renal, cardiovascular and hepatic

9 CLINICAL NETWORK

– Specialized and primary care units in Spain and Portugal

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