1 ANIMAL MODELS (mouse and rat)
– Renal diseases
- Acute kidney injury (toxic, ischemic, septic, pre-renal)
- Chronic kidney disease
- Fibrosis (renal mass reduction, ureteral obstruction)
– Hypertension
- Genetic
- Induced (DOCA-salt, L-NAME, aortic coarctation, AII pumps, renal artery clamping, Goldblatt)
– Diabetes
- Genetic
- Induced (streptozotocin)
– Other models
- Hepatic diseases
- Tumour models
- Wound healing
2 ISOLATED ORGAN
– In situ & ex vivo perfused kidney
– Isolated artery contraction (wire myograph)
– Isolated heart and atria
3 RENAL FUNCTION
– Glomerular filtration rate
- Inulin clearance
- Creatinine clearance
- Sinistrin clearance
– Renal plasma flow
- Laser doppler
- PAH clearance
- Microspheres
– Electrolyte balance
– Urinary and tissue biomarkers of renal injury
– Furosemide test
4 CARDIOVASCULAR FUNCTION
– Arterial pressure and heart rate
- Telemetry
- Sphygmomanometry
- Canulation
– Vascular function and stiffness
- Retinography
- ABI Index, PWV, IMT
- pAIX, cAIX, AASSI
– Electrocardiography and echocardiography
– Angiogenesis
5 CELLULAR & MOLECULAR BIOLOGY
– Cell culture
- Primary cultures
- Cell lines
– Flow cytometry
– Protein expression (Western blot, ELISA)
– mRNA analysis (qRT-PCR, digital RT-PCR)
– Live cell light and fluorescence microscopy
– Intracellular signalling
– Cell viability and cell cycle
-Cell death (apoptosis, necrosis, necroptosis)
– Cell contraction, migration and invasion
– Oxidative stress and inflammation
6 HISTOPATHOLOGY
– Basic stains
– Immunohistochemistry
– Immunofluorescence
7 PROTEOMICS & METABOLOMICS
– 2D-DIGE
– MALDI-TOF
– TOF-TOF instruments
– MS/MS
– PMF
– MALDI biotypes
– MALDI imaging
– HPLC, UPLC, FPLC
8 TOXICOLOGY
– In silico
– Preclinical
– Renal, cardiovascular and hepatic
9 CLINICAL NETWORK
– Specialized and primary care units in Spain and Portugal
