1 ANIMAL MODELS (mouse and rat)

– Renal diseases

• Acute kidney injury (toxic, ischemic, septic, pre-renal)
• Chronic kidney disease
• Fibrosis (renal mass reduction, ureteral obstruction)

– Hypertension

• Genetic
• Induced (DOCA-salt, L-NAME, aortic coarctation, AII pumps, renal artery clamping, Goldblatt)

– Diabetes

• Genetic
• Induced (streptozotocin)

– Other models

• Hepatic diseases
• Tumour models
• Wound healing

2 ISOLATED ORGAN

– In situ & ex vivo perfused kidney

– Isolated artery contraction (wire myograph)

– Isolated heart and atria

3 RENAL FUNCTION

– Glomerular filtration rate

• Inulin clearance
• Creatinine clearance
• Sinistrin clearance

– Renal plasma flow

• Laser doppler
• PAH clearance
• Microspheres

– Electrolyte balance

– Urinary and tissue biomarkers of renal injury

– Furosemide test

4 CARDIOVASCULAR FUNCTION

– Arterial pressure and heart rate

• Telemetry
• Sphygmomanometry
• Canulation

– Vascular function and stiffness

• Retinography
• ABI Index, PWV, IMT
• pAIX, cAIX, AASSI

– Electrocardiography and echocardiography

– Angiogenesis

5 CELLULAR & MOLECULAR  BIOLOGY

– Cell culture

• Primary cultures
• Cell lines

– Flow cytometry

– Protein expression (Western blot, ELISA)

– mRNA analysis (qRT-PCR, digital RT-PCR)

– Live cell light and fluorescence microscopy

– Intracellular signalling

– Cell viability and cell cycle

 -Cell death (apoptosis, necrosis, necroptosis)

– Cell contraction, migration and invasion

– Oxidative stress and inflammation

6 HISTOPATHOLOGY

– Basic stains

– Immunohistochemistry

– Immunofluorescence

7 PROTEOMICS & METABOLOMICS

– 2D-DIGE

– MALDI-TOF

– TOF-TOF instruments

– MS/MS

– PMF

– MALDI biotypes

– MALDI imaging

– HPLC, UPLC, FPLC

8 TOXICOLOGY

– In silico

– Preclinical

– Renal, cardiovascular and hepatic

9 CLINICAL NETWORK

– Specialized and primary care units in Spain and Portugal